Recent ProfessorsNot yet assignedRecent SemestersNo scheduled sections on recordClass SizeNot publishedCredits3PrerequisiteBIO 002 or BIO 014 Transfers ToCSUDescriptionCovers the principles of stem cell biology, cell therapies (like CAR-T treatments of cancer), and the production of virus vectors for gene therapies. Topics include the technological and regulatory considerations required to generate immune cells or stem cells as therapy agents and the manufacturing techniques used to generate virus vectors for gene therapy or to transduce cells. This includes the growth of embryonic stem cells, adult stem cells, induced pluripotent stem cells, and the ethical issues involved in stem cell research. Emphasis on laboratory techniques including culture and analysis of cells, and the use of several methods of cell culture used in the production of viruses. BIOT 066 4.0 Units Principles and Practice of Biomanufacturing Provides conceptual knowledge and hands-on training related to the manufacture of biological molecules using genetically engineered cells. Students will learn cell culture techniques that include working under aseptic conditions, sterile techniques, media preparation, cell and molecule quantification, and the scale-up of cell cultures. Students will inoculate, monitor growth, and harvest cultures from bioreactors. This course also covers protein recovery, concentration, and purification techniques including sample preparation, tangential flow filtration, column chromatograph, and the use of assays for purification analysis. Students will become familiar with the regulatory environment of biomanufacturing including current Good Manufacturing Process (cGMP), Standard Operating Procedures (SOP's), and production batch records. 