TransfermaticBIOT at Ohlone College

    BIOT 111B - PCR Primer Design and Optimization and Reverse

    Recent Professors
    Not yet assigned
    Recent Semesters
    No scheduled sections on record
    Class Size
    Not published
    Credits
    1
    Prerequisite
    BIOL-101A or BIOT-110A
    Transfers To
    CSU
    Description
    Accepted For Credit: CSU Offered: Fall Semester, Every Year This course combines lecture and lab approaches to teaching students advanced topics in PCR, including BLAST searches and DNA alignment protocols for locating minimal variable sequences to use in constructing PCR primers, principles of primer design, and optimization techniques for PCR reactions. Students design primers; optimize salt and temperature parameters for PCR; and perform RT-PCR. Students are required to buy a lab coat, goggles, and examination gloves for use in this course. (GR)