Transfers ToCSUDescriptionThis course teaches students how to produce, purify, and analyze useful biological molecules, including recombinant proteins. Students will grow organisms for production of biomolecules under carefully controlled and monitored conditions, harvest biomass, and perform extractions. Purification steps include sample preparation, determination of best biomolecule separation method, column chromatography, and large-scale recovery. Analysis of product recovery includes qualitative and quantitative assays, standard curves, and determination of yield. Students isolate a specific protein from a complex cell lysate using affinity and other forms of chromatography, and analyze the results by qualitative and quantitative protein assays. The size and purity of the isolated protein are analyzed by SDS-Polyacrylamide Gel Electrophoresis (SDS-PAGE). Current Good Manufacturing Process (cGMP), Good Laboratory Practice (GLP), and Standard Operating Procedures (SOP's) in relation to these techniques are used throughout. 